FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAAFRAAPEI-208_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAAFRAAPEI-208_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34313565
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGTTCCTGGTGCTCCTTGGAGCTGCCTTTGCTCTGGATGATGAC464780.1354508049513363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG66800.019.1326584
AATCGGT73500.017.5980935
AGAATCG74600.017.1321413
CGTACTA16200.014.25869135
TCGGTTT91350.014.0149027
ACAATCG20550.013.7016383
CCGGGAT16150.013.664621
GTTCAAT122900.013.1979221
CGGCGAT69350.012.9832611
TTAAGCG30900.012.9565247
TCGCTAG8750.012.821433
TGCCGTA89950.012.81516331
ATCGGTT100450.012.5919666
CGGTTTT101350.012.588678
CAAGAAT121800.012.5561371
GATATAT59650.012.3540685
CGCCCAT24750.012.3047831
CAATCGG15550.012.1658854
CCTAAGG21200.012.03637914
CCGGTAT10000.011.9169151
Produced by FastQC (version 0.11.5)